Fmoc-Leu-Wang Resin LL (100–200 Mesh): Preloaded Resin for C-Terminal Leucine Peptides

Fmoc-Leu-Wang Resin LL (100–200 mesh) is a low-loading (LL), preloaded solid-phase resin used in Fmoc-based peptide synthesis. It features Fmoc-protected leucine covalently bound via an ester linkage to a Wang linker, which is anchored on a polystyrene-divinylbenzene (PS-DVB) matrix. The Wang linker enables peptide synthesis with a C-terminal carboxylic acid, while the Fmoc-Leu group provides a convenient starting point for chain elongation.

The low-loading format (~0.2–0.4 mmol/g) is optimized for the synthesis of long or aggregation-prone sequences, reducing on-resin crowding and improving coupling efficiency.

Specifications

• Matrix: Polystyrene cross-linked with ~1% DVB

• Linker: Wang (acid-labile)

• Preloaded Amino Acid: Fmoc-Leucine (Fmoc-Leu-OH)

• Loading: Low Loading (LL), typically 0.2–0.4 mmol/g

• Particle Size: 100–200 mesh (approx. 75–150 µm)

• Cleavage: Acidolytic, typically 95% TFA with scavengers

• Solvent Compatibility: Swells well in DCM, DMF, NMP, etc.

• C-Terminal Product: Free carboxylic acid (-COOH)

Key Features

• Preloaded with Fmoc-Leu: Reduces setup time, simplifies synthesis

• Low Loading: Minimizes steric hindrance for improved yield and purity of longer sequences

  C-Terminal Acid: Ideal for biological relevance and structure-activity studies

• Fmoc-Compatible: Seamlessly integrates into standard SPPS protocols

• Suitable for Automation or Manual Synthesis

Applications

• Synthesis of peptides with C-terminal leucine and free acid

• Therapeutic peptide development

• Structure-activity relationship (SAR) studies involving leucine termini

• Long, hydrophobic, or aggregation-prone sequences

• Peptide libraries and screening assays

Cleavage & Deprotection

• Cleavage Conditions: 95% TFA + 2.5% water + 2.5% TIS (or similar)

• Time: 1–3 hours at room temperature

• Result: Fully deprotected peptide with C-terminal –COOH

Handling & Storage

• Store dry, in the dark, and away from moisture

• Pre-swell in suitable solvents (e.g., DMF, DCM) before use

• Compatible with microwave and room-temperature SPPS protocols

Comparison With Related Resins

Conclusion

Fmoc-Leu-Wang Resin LL (100–200 mesh) is a reliable, preloaded solid support for the efficient synthesis of C-terminally free acid peptides, particularly where leucine is the terminal residue. Its low-loading format enhances synthesis performance for challenging sequences, ensuring high yield and purity in both manual and automated workflows.

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